Belief in public efficacy, trust and attitudes to modern genetic science

The official published version can be accessed from the links below - Copyright @ 2007 Wiley-BlackwellGovernment and policymakers want to engage the public in a dialogue about the conduct and consequences of science and increasingly seek to actively involve citizens in decision-making processes. Implicit in this thinking is that greater transparency and public inclusion will help dispel fears associated with new scientific advancements, foster greater public trust in those accountable, and ultimately increase the acceptability of new technologies. Less understood, however, are public perceptions about such high-level involvement in science and how these map onto public trust and attitudes within a diverse population. This article uses the concept of public efficacy—the extent to which people believe that the public might be able to affect the course of decision making—to explore differences in trust, attentiveness, and attitudes toward modern genetic science. Using nationally representative data from the 2003 British Social Attitudes Survey, we begin by examining the characteristics of those who have a positive belief about public involvement in this area of scientific inquiry. We then focus on how this belief maps on to indicators of public trust in key stakeholder groups, including the government and genetic scientists. Finally, we consider the relationship between public efficacy and trust and attitudes toward different applications of genetic technology. Our findings run contrary to assumptions that public involvement in science will foster greater trust and lead to a climate of greater acceptance for genetic technology. A belief in public efficacy does not uniformly equate with more trusting attitudes toward stakeholders but is associated with less trust in government rules. Whereas trust is positively correlated with more permissive attitudes about technologies such as cloning and gene therapy, people who believe in high-level public involvement are less likely to think that these technologies should be allowed than those who do not.The support of the Economics and Social Research Council (ESRC) is acknowledged. The work arises from the ESRC Attitudes to Genomics project L145251005

An initial event in insect innate immune response: structural and biological studies of interactions between β-1,3-glucan and the N-terminal domain of β-1,3-glucan recognition protein

In response to invading microorganisms, insect β-1,3-glucan recognition protein (βGRP), a soluble receptor in the hemolymph, binds to the surfaces of bacteria and fungi and activates serine protease cascades that promote destruction of pathogens by means of melanization or expression of antimicrobial peptides. Here we report on the NMR solution structure of the N-terminal domain of βGRP (N-βGRP) from Indian meal moth (Plodia interpunctella), which is sufficient to activate the prophenoloxidase (proPO) pathway resulting in melanin formation. NMR and isothermal calorimetric titrations of N-βGRP with laminarihexaose, a glucose hexamer containing β-1,3 links, suggest a weak binding of the ligand. However, addition of laminarin, a glucose polysaccharide (~ 6 kDa) containing β-1,3 and β-1,6 links that activates the proPO pathway, to N-βGRP results in the loss of NMR cross-peaks from the backbone 15N-1H groups of the protein, suggesting the formation of a large complex. Analytical ultra centrifugation (AUC) studies of formation of N-βGRP:laminarin complex show that ligand-binding induces sel-fassociation of the protein:carbohydrate complex into a macro structure, likely containing six protein and three laminarin molecules (~ 102 kDa). The macro complex is quite stable, as it does not undergo dissociation upon dilution to sub-micromolar concentrations. The structural model thus derived from the present studies for N-βGRP:laminarin complex in solution differs from the one in which a single N-βGRP molecule has been proposed to bind to a triple helical form of laminarin on the basis of an X-ray crystallographic structure of N-βGRP:laminarihexaose complex [Kanagawa, M., Satoh, T., Ikeda, A., Adachi, Y., Ohno, N., and Yamaguchi, Y. (2011) J. Biol. Chem. 286, 29158-29165]. AUC studies and phenoloxidase activation measurements carried out with the designed mutants of N-βGRP indicate that electrostatic interactions involving Asp45, Arg54, and Asp68 between the ligand-bound protein molecules contribute in part to the stability of N-βGRP:laminarin macro complex and that a decreased stability is accompanied by a reduced activation of the proPO pathway. Increased β-1,6 branching in laminarin also results in destabilization of the macro complex. These novel findings suggest that ligand-induced self-association of βGRP:β-1,3-glucan complex may form a platform on a microbial surface for recruitment of downstream proteases, as a means of amplification of the initial signal of pathogen recognition for the activation of the proPO pathway

Refining Chandra/ACIS Subpixel Event Repositioning Using a Backside Illuminated CCD Model

Subpixel event repositioning (SER) techniques have been demonstrated to significantly improve the already unprecedented spatial resolution of Chandra X-ray imaging with the Advanced CCD Imaging Spectrometer (ACIS). Chandra CCD SER techniques are based on the premise that the impact position of events can be refined, based on the distribution of charge among affected CCD pixels. ACIS SER models proposed thus far are restricted to corner split (3- and 4-pixel) events, and assume that such events take place at the split pixel corners. To improve the event counting statistics, we modified the ACIS SER algorithms to include 2-pixel split events and single pixel events, using refined estimates for photon impact locations. Furthermore, simulations that make use of a high-fidelity backside illuminated (BI) CCD model demonstrate that mean photon impact positions for split events are energy dependent leading to further modification of subpixel event locations according to event type and energy, for BI ACIS devices. Testing on Chandra CCD X-ray observations of the Orion Nebula Cluster indicates that these modified SER algorithms further improve the spatial resolution of Chandra/ACIS, to the extent that the spreading in the spatial distribution of photons is dominated by the High Resolution Mirror Assembly, rather than by ACIS pixelization.Comment: 23 pages, 8 figures, 2nd version, submitted to Ap